AutoPrime: Selecting Primers for Expressed Sequences
Gunnar Wrobel, Felix Kokocinski, Peter LichterGenome Research 2004 5:P11
Primers designed for expressed sequences should be
specific for the mRNA sequence of a gene but not
yield a product from its genomic sequence. Already
slight contaminations of genomic DNA can lead to incorrect
measurements for sensitive methods like realtime
PCR (RT-PCR). The software AutoPrime automates
the tedious task of generating such primers
for RT-PCR experiments by combining the benefits
of the ensembl database with the primer3 software.
It can be used as a command-line tool using the language
perl or as a web-based application.
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